Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis (Record no. 75384)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03438nam a22005535i 4500 |
| 001 - CONTROL NUMBER | |
| control field | 978-981-10-4753-4 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220801213615.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 170720s2018 si | s |||| 0|eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| ISBN | 9789811047534 |
| -- | 978-981-10-4753-4 |
| 082 04 - CLASSIFICATION NUMBER | |
| Call Number | 610.28 |
| 100 1# - AUTHOR NAME | |
| Author | Zhuang, Bin. |
| 245 10 - TITLE STATEMENT | |
| Title | Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. 2018. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | XXIII, 114 p. 58 illus., 44 illus. in color. |
| 490 1# - SERIES STATEMENT | |
| Series statement | Springer Theses, Recognizing Outstanding Ph.D. Research, |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Remark 2 | Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | https://doi.org/10.1007/978-981-10-4753-4 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | eBooks |
| 264 #1 - | |
| -- | Singapore : |
| -- | Springer Nature Singapore : |
| -- | Imprint: Springer, |
| -- | 2018. |
| 336 ## - | |
| -- | text |
| -- | txt |
| -- | rdacontent |
| 337 ## - | |
| -- | computer |
| -- | c |
| -- | rdamedia |
| 338 ## - | |
| -- | online resource |
| -- | cr |
| -- | rdacarrier |
| 347 ## - | |
| -- | text file |
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| -- | rda |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biomedical engineering. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biotechnology. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biomaterials. |
| 650 #0 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Nucleic acids. |
| 650 14 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Biomedical Engineering and Bioengineering. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Chemical Bioengineering. |
| 650 24 - SUBJECT ADDED ENTRY--SUBJECT 1 | |
| -- | Nucleic Acid. |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| -- | 2190-5061 |
| 912 ## - | |
| -- | ZDB-2-ENG |
| 912 ## - | |
| -- | ZDB-2-SXE |
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