Central Library

Single-particle cryo-EM of biological macromolecules / (Record no. 82810)

000 -LEADER
fixed length control field 05548nam a2200721 i 4500
001 - CONTROL NUMBER
control field 9780750330398
003 - CONTROL NUMBER IDENTIFIER
control field IOP
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230516170223.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field m eo d
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field cr cn |||m|||a
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210611s2021 enka fob 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9780750330398
Qualifying information ebook
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9780750330381
Qualifying information mobi
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN 9780750330374
Qualifying information print
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN 9780750330404
Qualifying information myPrint
024 7# - OTHER STANDARD IDENTIFIER
Standard number or code 10.1088/978-0-7503-3039-8
Source of number or code doi
035 ## - SYSTEM CONTROL NUMBER
System control number (CaBNVSL)thg00082502
035 ## - SYSTEM CONTROL NUMBER
System control number (OCoLC)1259501142
040 ## - CATALOGING SOURCE
Original cataloging agency CaBNVSL
Language of cataloging eng
Description conventions rda
Transcribing agency CaBNVSL
Modifying agency CaBNVSL
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QP801.P64
Item number S567 2021eb
072 #7 - SUBJECT CATEGORY CODE
Subject category code PHVN
Source bicssc
072 #7 - SUBJECT CATEGORY CODE
Subject category code SCI009000
Source bisacsh
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 574.19/283
Edition number 23
245 00 - TITLE STATEMENT
Title Single-particle cryo-EM of biological macromolecules /
Statement of responsibility, etc. edited by Robert M. Glaeser, Eva Nogales, Wah Chiu.
264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE
Place of production, publication, distribution, manufacture Bristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) :
Name of producer, publisher, distributor, manufacturer IOP Publishing,
Date of production, publication, distribution, manufacture, or copyright notice [2021]
300 ## - PHYSICAL DESCRIPTION
Extent 1 online resource (various pagings) :
Other physical details illustrations (some color).
336 ## - CONTENT TYPE
Content type term text
Source rdacontent
337 ## - MEDIA TYPE
Media type term electronic
Source isbdmedia
338 ## - CARRIER TYPE
Carrier type term online resource
Source rdacarrier
490 1# - SERIES STATEMENT
Series statement [IOP release $release]
490 1# - SERIES STATEMENT
Series statement Biophysical Society-IOP series
490 1# - SERIES STATEMENT
Series statement IOP ebooks. [2021 collection]
500 ## - GENERAL NOTE
General note "Version: 20210205"--Title page verso.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references.
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note 1. Introduction and overview -- 1.1. Visualizing biological molecules to understand life's principles -- 1.2. Recovery of 3D structures from images of weak-phase objects
505 8# - FORMATTED CONTENTS NOTE
Formatted contents note 2. Sample preparation -- 2.1. Overview -- 2.2. Initial screening of samples in negative stain -- 2.3. Standard method of making grids for cryo-EM -- 2.4. Requirement to make very thin specimens for cryo-EM -- 2.5. Current strategies for optimizing preparation of cryo-grids
505 8# - FORMATTED CONTENTS NOTE
Formatted contents note 3. Data collection -- 3.1. Overview -- 3.2. Radiation damage in cryo-EM -- 3.3. Low-dose protocols for recording images -- 3.4. Practical considerations : defocus, stigmation, coma-free illumination, and phase plates -- 3.5. Practical considerations : movie-mode data acquisition
505 8# - FORMATTED CONTENTS NOTE
Formatted contents note 4. Data processing -- 4.1. Overview -- 4.2. Automated extraction of particles -- 4.3. CTF estimation and image correction (restoration) -- 4.4. Merging data from structurally homogeneous subsets -- 4.5. 3D classification of structurally heterogeneous particles -- 4.6. Preferred orientation : how to recognize and deal with adverse effects -- 4.7. B factors and map sharpening -- 4.8. Optical aberrations and Ewald sphere curvature
505 8# - FORMATTED CONTENTS NOTE
Formatted contents note 5. Map validation -- 5.1. Overview -- 5.2. Measures of resolution : FSC and local resolution -- 5.3. Recognizing the effect of bias and over-fitting -- 5.4. Estimates of alignment accuracy -- 5.5. Discussion
505 8# - FORMATTED CONTENTS NOTE
Formatted contents note 6. Model building and validation -- 6.1. Overview -- 6.2. Using known components or homologs : model building -- 6.3. Building atomistic models in cryo-EM density maps -- 6.4. Quality evaluation of cryo-EM map-derived models -- 6.5. How algorithms from crystallography are helping electron cryo-microscopy -- 6.6. Archiving structures and data.
520 3# - SUMMARY, ETC.
Summary, etc. This edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. It is especially well suited for larger complexes and molecular machines. This book provides a comprehensive, accessible and authoritative introduction to the field. It covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data collection, data analysis, and the final validation of results.
521 ## - TARGET AUDIENCE NOTE
Target audience note Beginning scientists in academia, pharma, and biotech.
530 ## - ADDITIONAL PHYSICAL FORM AVAILABLE NOTE
Additional physical form available note Also available in print.
538 ## - SYSTEM DETAILS NOTE
System details note Mode of access: World Wide Web.
538 ## - SYSTEM DETAILS NOTE
System details note System requirements: Adobe Acrobat Reader, EPUB reader, or Kindle reader.
545 ## - BIOGRAPHICAL OR HISTORICAL DATA
Biographical or historical data Professor Glaeser (UC Berkeley) made major contributions to the initial development of cryo-EM technology, and he currently is active in the improvement of specimen preparation. Professor Nogales (UC Berkeley) has used cryo-EM throughout her scientific career, successfully applying this technique for the last three decades to systems that defied structural characterization. Professor Chiu (Stanford University) developed early experimental and computational methods toward 3D structure determination of biomolecules toward atomic resolution.
588 0# - SOURCE OF DESCRIPTION NOTE
Source of description note Title from PDF title page (viewed on June 11, 2021).
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Macromolecules
General subdivision Analysis.
9 (RLIN) 70271
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Biomolecules
General subdivision Analysis.
9 (RLIN) 12076
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element High resolution imaging.
9 (RLIN) 11219
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Biophysics.
Source of heading or term bicssc
9 (RLIN) 4093
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element SCIENCE / Life Sciences / Biophysics.
Source of heading or term bisacsh
9 (RLIN) 14896
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Glaeser, Robert M.,
Relator term author.
9 (RLIN) 70272
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Nogales, Eva,
Relator term author.
9 (RLIN) 70273
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Chiu, Wah,
Relator term author.
9 (RLIN) 70274
710 2# - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Institute of Physics (Great Britain),
Relator term publisher.
9 (RLIN) 11622
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Print version:
International Standard Book Number 9780750330374
-- 9780750330404
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title IOP (Series).
Name of part/section of a work Release 21.
9 (RLIN) 70275
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Biophysical Society-IOP series.
9 (RLIN) 70276
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title IOP ebooks.
Name of part/section of a work 2021 collection.
9 (RLIN) 70277
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://iopscience.iop.org/book/978-0-7503-3039-8">https://iopscience.iop.org/book/978-0-7503-3039-8</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type eBooks

No items available.