Modelling and Analysis -- Rate Equations For Graphs -- Stationary Distributions and Metastable Behaviour for Self-Regulating Proteins with General Lifetime Distributions -- Accelerating Reactions at the DNA Can Slow Down Transient Gene Expression -- Graphical Conditions for Rate Independence in Chemical Reaction Networks -- Interval Constraint Satisfaction and Optimization for Biological Homeostasis and Multistationarity -- Growth Dependent Computation of Chokepoints in Metabolic Networks -- On the Complexity of Quadratization for Polynomial Differential Equations -- Comparing Probabilistic and Logic Programming Approaches to Predict the Effects of Enzymes in a Neurogenerative Disease Model -- Boolean Networks -- Control Strategy Identification via Trap Spaces in Boolean Networks -- Qualitative Analysis of Mammalian Circadian Oscillations: Cycle Dynamics and Robustness -- Synthesis and Simulation of Ensembles of Boolean Networks for Cell Fate Decision -- Classifier Construction in Boolean Networks Using Algebraic Methods -- Sequential Temporary and Permanent Control of Boolean Networks -- Inference and Identification -- ABC(SMC)^2: Simultaneous Inference and Model Checking of Chemical Reaction Networks -- Parameter Synthesis for Hybrid Systems from Hybrid CTL Specifications -- Core Models of Receptor Reactions Evaluate Basic Pathway Designs Enabling Heterogeneous Commitments to Apoptosis -- Drawing the Line: Basin Boundaries in Safe Petri Nets -- Tools -- ModRev - Model Revision Tool for Boolean Logical Models of Biological Regulatory Networks -- fnyzer: a Python Package for the Analysis of Flexible Nets -- eBCSgen: A Software Tool for Biochemical Space Language -- What is a Cell Cycle Checkpoint ? The TotemBioNet Answer -- Kaemika App, Integrating Protocols and Chemical Simulation -- Tutorials -- Tutorial: The CoLoMoTo Interactive Notebook, Accessible and Reproducible Computational Analyses for Qualitative Biological Networks -- Integrating Experimental Pharmacology and Systems Biology for GPCR Drug Discovery.

This book constitutes the refereed proceedings of the 18th International Conference on Computational Methods in Systems Biology, CMSB 2020, held in Konstanz, Germany, in September 2020.* The 17 full papers and 5 tool papers were carefully reviewed and selected from 30 submissions. In addition 3 abstracts of invited talks and 2 tutorials have been included in this volume. Topics of interest include formalisms for modeling biological processes; models and their biological applications; frameworks for model verification, validation, analysis, and simulation of biological systems; high-performance computational systems biology and parallel implementations; model inference from experimental data; model integration from biological databases; multi-scale modeling and analysis methods; computational approaches for synthetic biology; and case studies in systems and synthetic biology. * The conference was held virtually due to the COVID-19 pandemic.