Technology platforms for 3D cell culture : a user's guide /
edited by Stefan Przyborski.
- 1 online resource (xviii, 406 pages)
Includes bibliographical references and index.
Title Page ; Copyright Page; Contents; List of contributors; Preface; List of abbreviations; Chapter 1 An introduction to the third dimension for routine cell culture ; Introduction; Aggregate-based technologies ; Scaffold-based technologies ; 3D bioreactors; Barriers to adoption and future directions; References; Part I Aggregate-based technologies ; Chapter 2 Gravity-enforced microtissue engineering ; Introduction; Product description; Tumour microtissue size profiling for drug testing; Conclusions; Troubleshooting and notes (Table 2.3); Summary; References Chapter 3 Physiologically relevant spheroid models for three-dimensional cell culture Introduction; Description of technology; Experimental results obtained using the Perfecta3D hanging drop plates; Troubleshooting; Conclusion; References; Chapter 4 NanoCulture Plate: A scaffold-based high-throughput three-dimensional cell culture system suitable for live imaging and co-culture ; Introduction; Description of technology; Example of application; Troubleshooting; Summary; References; Chapter 5 Micro-moulded non-adhesive hydrogels to form multicellular microtissues -- the 3D Petri Dish® IntroductionDescription of technology; Applications; Protocols; Troubleshooting; Conclusion; References; Chapter 6 Organotypic microtissues on an air-liquid interface ; Introduction; Description of technology; 3D neural OrganDOT" tissues for neurotoxicology studies; Troubleshooting; References; Part II Hydrogels; Chapter 7 Materials and assay systems used for three?dimensional cell culture; Introduction; Description of technology; Applications of 3D cultures in vitro; Conclusion; References; Chapter 8 HyStem®, a customisable hyaluronan?based hydrogel matrix for 3D cell culture; Introduction Description of technologyApplications; Troubleshooting; Conclusion; Appendix; References; Chapter 9 3-D Life biomimetic hydrogels: A modular system for cell environment design ; Introduction; Applications; Description of technology; A tumour-stroma model: 3D co-culture of MCF-7 cells and primary fibroblasts ; Troubleshooting; Conclusion; References; Part III Scaffolds; Chapter 10 Alvetex®, a highly porous polystyrene scaffold for routine three-dimensional cell culture ; Introduction; Description of technology; Example applications of Alvetex Scaffold technology; Troubleshooting; Conclusion