000 03831nam a22005535i 4500
001 978-3-642-34094-9
003 DE-He213
005 20200421112221.0
007 cr nn 008mamaa
008 121116s2013 gw | s |||| 0|eng d
020 _a9783642340949
_9978-3-642-34094-9
024 7 _a10.1007/978-3-642-34094-9
_2doi
050 4 _aR856-857
072 7 _aMQW
_2bicssc
072 7 _aTEC009000
_2bisacsh
082 0 4 _a610.28
_223
100 1 _aSong, Kwon-Ho.
_eauthor.
245 1 0 _aSialo-Xenoantigenic Glycobiology
_h[electronic resource] :
_bMolecular Glycobiology of Sialylglycan-Xenoantigenic Determinants in Pig to Human Xenotransplantation /
_cby Kwon-Ho Song, Cheorl-Ho Kim.
264 1 _aBerlin, Heidelberg :
_bSpringer Berlin Heidelberg :
_bImprint: Springer,
_c2013.
300 _aXVI, 64 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aSungkyunkwan University Outstanding Research,
_x2195-3546 ;
_v1
505 0 _aScreening of pig glycosyltransferase genes related to xenoantigens and their masking -- Cloning and tissue specific expression of pcmah and its alternative transcripts -- Functional characterization of pCMAH in the synthesis of N-glycolylneuraminic acid as the xenoantigenic determinant in pig-to-human xenotransplantation -- Identification of two alternative promoters of the pig CMP-N-acetylneuraminic acid hydroxylase gene -- Screening for xenoantigenic determinants formed by sialyltransferases.
520 _aCarbohydrate antigens on glycoconjugates of mammalian cells play crucial roles in various biological processes and are epitopes recognized by the immune system, as glycobiology has hugely been progressed during the past two decades. The book focuses on sialic acid-based xenoantigenes. In pig to human xenotransplantation, exposure of pig organs to human blood results in hyper acute rejection (HAR), caused by differences in carbohydrate epitopes between human and pig vascular endothelia. Although Gal-antigen as major antigen was eliminated, the remaining non-Gal antigens are considered to be xenoantigens. Sialosyl-Tn or Hanganutziu-Deicher (HD), are non-Gal antigens specific to natural antibodies in human. To overcome rejection responses such as HAR, studies of genes involved in carbohydrate antigens, causing xenoantigenicity, are necessary. Knowledge of pig glycosyltransferases are also useful to apply to xenoantigen masking or identification of the xenoantigenic sialylglycan(s). In the first chapter the screening for pig glycosyltransferase genes for xenoantigens is presented. In the chapter II to IV the cloning, characterization, and investigation of the regulatory mechanism of the pig CMAH gene in NeuGc biosynthesis is shown. Lastly, the effects of an alteration of pig glycosylation patterns on human serum-mediated cytotoxicity, caused by human sialyltransferases including hST6GalNAc IV is presented.  .
650 0 _aEngineering.
650 0 _aHuman genetics.
650 0 _aMolecular biology.
650 0 _aMedical biochemistry.
650 0 _aBiochemistry.
650 0 _aBiomedical engineering.
650 1 4 _aEngineering.
650 2 4 _aBiomedical Engineering.
650 2 4 _aMolecular Medicine.
650 2 4 _aHuman Genetics.
650 2 4 _aMedical Biochemistry.
650 2 4 _aBiochemistry, general.
700 1 _aKim, Cheorl-Ho.
_eauthor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9783642340932
830 0 _aSungkyunkwan University Outstanding Research,
_x2195-3546 ;
_v1
856 4 0 _uhttp://dx.doi.org/10.1007/978-3-642-34094-9
912 _aZDB-2-ENG
942 _cEBK
999 _c57421
_d57421