| 000 | 03632nam a22005295i 4500 | ||
|---|---|---|---|
| 001 | 978-3-031-02570-9 | ||
| 003 | DE-He213 | ||
| 005 | 20240730164004.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 220601s2011 sz | s |||| 0|eng d | ||
| 020 |
_a9783031025709 _9978-3-031-02570-9 |
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| 024 | 7 |
_a10.1007/978-3-031-02570-9 _2doi |
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| 050 | 4 | _aQA1-939 | |
| 072 | 7 |
_aPB _2bicssc |
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_aMAT000000 _2bisacsh |
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_aPB _2thema |
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_a510 _223 |
| 100 | 1 |
_aMeer, Jan Roelof. _eauthor. _4aut _4http://id.loc.gov/vocabulary/relators/aut _981524 |
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| 245 | 1 | 0 |
_aBacterial Sensors _h[electronic resource] : _bSynthetic Design and Application Principles / _cby Jan Roelof Meer. |
| 250 | _a1st ed. 2011. | ||
| 264 | 1 |
_aCham : _bSpringer International Publishing : _bImprint: Springer, _c2011. |
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| 300 |
_aXIII, 153 p. _bonline resource. |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 347 |
_atext file _bPDF _2rda |
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| 490 | 1 |
_aSynthesis Lectures on Synthetic Biology, _x2151-0016 |
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| 505 | 0 | _aShort History of the use of Bacteria for Biosensing and Bioreporting -- Genetic Engineering Concepts -- Measuring with Bioreporters -- Epilogue. | |
| 520 | _aBacterial reporters are live, genetically engineered cells with promising application in bioanalytics. They contain genetic circuitry to produce a cellular sensing element, which detects the target compound and relays the detection to specific synthesis of so-called reporter proteins (the presence or activity of which is easy to quantify). Bioassays with bacterial reporters are a useful complement to chemical analytics because they measure biological responses rather than total chemical concentrations. Simple bacterial reporter assays may also replace more costly chemical methods as a first line sample analysis technique. Recent promising developments integrate bacterial reporter cells with microsystems to produce bacterial biosensors. This lecture presents an in-depth treatment of the synthetic biological design principles of bacterial reporters, the engineering of which started as simple recombinant DNA puzzles, but has now become a more rational approach of choosing and combining sensing, controlling and reporting DNA 'parts'. Several examples of existing bacterial reporter designs and their genetic circuitry will be illustrated. Besides the design principles, the lecture also focuses on the application principles of bacterial reporter assays. A variety of assay formats will be illustrated, and principles of quantification will be dealt with. In addition to this discussion, substantial reference material is supplied in various Annexes. Table of Contents: Short History of the use of Bacteria for Biosensing and Bioreporting / Genetic Engineering Concepts / Measuring with Bioreporters / Epilogue. | ||
| 650 | 0 |
_aMathematics. _911584 |
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| 650 | 0 |
_aEngineering. _99405 |
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| 650 | 0 |
_aBiotechnology. _97533 |
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| 650 | 0 |
_aBiology _xTechnique. _981520 |
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| 650 | 1 | 4 |
_aMathematics. _911584 |
| 650 | 2 | 4 |
_aTechnology and Engineering. _981525 |
| 650 | 2 | 4 |
_aBiotechnology. _97533 |
| 650 | 2 | 4 |
_aBiological Techniques. _943339 |
| 710 | 2 |
_aSpringerLink (Online service) _981526 |
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| 773 | 0 | _tSpringer Nature eBook | |
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_iPrinted edition: _z9783031014420 |
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_iPrinted edition: _z9783031036989 |
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_aSynthesis Lectures on Synthetic Biology, _x2151-0016 _981527 |
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| 856 | 4 | 0 | _uhttps://doi.org/10.1007/978-3-031-02570-9 |
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| 942 | _cEBK | ||
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